Human Proliferation Associated Protein 2G4 (PA2G4) ELISA Kit
The Human PA2G4 (Proliferation Associated Protein 2G4) ELISA Kit is a precision tool for the precise measurement of PA2G4 levels in human samples, including serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results that are invaluable for a variety of research endeavors.PA2G4, also known as Ebp1, is a critical protein involved in cell proliferation regulation, cell growth, and cell cycle progression.
Its dysregulation has been linked to various diseases, including cancer, making it a key biomarker for studying these conditions and potentially identifying new therapeutic strategies.With the Human PA2G4 ELISA Kit, researchers can confidently explore the role of PA2G4 in disease development and progression, paving the way for innovative treatment approaches and a deeper understanding of cellular proliferation mechanisms.
Product Name:
Human Proliferation Associated Protein 2G4 (PA2G4) ELISA Kit
Product Code:
HUDL02165
Size:
96 Assays
Target:
PA2G4
Synonyms:
EBP1, HG4-1, p38-2G4, Cell cycle protein p38-2G4 homolog, ErbB3-binding protein 1
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
