The PAF ELISA Kit is designed for the quantitative detection of Platelet Activating Factor (PAF) in various human biological samples. PAF is a crucial bioactive lipid that plays a significant role in numerous biological activities. It mediates platelet aggregation and degranulation, promotes inflammation, and participates in processes such as hemostasis, thrombosis, and allergic reactions. Accurate measurement of PAF is critical for understanding its mechanisms in disease conditions and for the development of therapeutic strategies. Assay Genie's PAF ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an excellent choice for both research and clinical applications. Trust Assay Genie's PAF ELISA Kit for accurate and dependable quantification of this crucial biomarker in your studies.
Product Name:
Human PAF (Platelet Activating Factor) ELISA Kit
SKU:
AEES00033
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
