Human Parathyroid Hormone Receptor 1 (PTHR1) ELISA Kit
The Human Parathyroid Hormone Receptor 1 (PTHr1) ELISA Kit is a reliable and accurate tool for measuring PTHr1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and precise results for a variety of research applications.PTHr1 is a key receptor involved in mediating the effects of parathyroid hormone, playing a crucial role in regulating calcium and phosphate levels in the body.
Dysregulation of PTHr1 signaling has been linked to various disorders such as osteoporosis, hyperparathyroidism, and renal diseases, making it a valuable biomarker for understanding these conditions and exploring potential therapeutic interventions.Overall, the Human PTHr1 ELISA Kit provides researchers with a powerful tool to study the role of PTHr1 in health and disease, offering valuable insights into its mechanisms and potential clinical implications.
Product Name:
Human Parathyroid Hormone Receptor 1 (PTHR1) ELISA Kit
Product Code:
HUDL02450
Size:
96 Assays
Target:
PTHR1
Synonyms:
PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
