The Human Procalcitonin (PCT) ELISA Kit is specifically designed for the precise measurement of procalcitonin levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, allowing for consistent and accurate results, making it suitable for various research purposes.Procalcitonin is a vital marker for detecting bacterial infections and sepsis, as its levels significantly rise in response to these conditions. Monitoring procalcitonin levels can aid in the early diagnosis and management of infections, guiding antibiotic therapy decisions and improving patient outcomes.Overall, the Human Procalcitonin (PCT) ELISA Kit is an invaluable tool for studying infectious diseases, sepsis, and inflammatory conditions, providing researchers with a reliable method for assessing procalcitonin levels in biological samples.
Product Name:
Human PCT (Procalcitonin) ELISA Kit
SKU:
HUES02515
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
