Human PEDF (Pigment Epithelium Derived Factor) ELISA Kit
The Human PEDF (Pigment Epithelium-Derived Factor) ELISA Kit is a cutting-edge tool for precise measurement of PEDF levels in human samples including serum, plasma, and cell culture supernatants. This advanced kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.PEDF is a vital protein known for its role in regulating angiogenesis, cell growth, and survival. Dysregulation of PEDF has been linked to various diseases such as cancer, diabetic retinopathy, and age-related macular degeneration, making it a crucial biomarker for investigating these conditions and developing potential treatments.Order the Human PEDF ELISA Kit today and unlock the potential for groundbreaking discoveries in the field of biomedical research.
Product Name:
Human PEDF (Pigment Epithelium Derived Factor) ELISA Kit
SKU:
HUES02826
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
