The Human Pentraxin-3 (PTX3) ELISA Kit is a powerful tool for accurately measuring levels of PTX3 in human biological samples such as serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring that researchers can obtain reliable and reproducible results for a wide range of research applications.Pentraxin-3 is a key protein involved in the immune response and inflammation, playing a critical role in various diseases and conditions such as cardiovascular diseases, infectious diseases, and autoimmune disorders.
By measuring PTX3 levels, researchers can gain valuable insights into the pathophysiology of these conditions and potentially identify new therapeutic targets.With its advanced technology and easy-to-use format, the Human Pentraxin-3 ELISA Kit is a must-have for any researcher looking to study PTX3 and its role in health and disease. Trust Assay Genie for high-quality ELISA kits that deliver accurate and dependable results every time.
Product Name:
Human Pentraxin 3, Long (PTX3) ELISA Kit
Product Code:
HUDL02465
Size:
96 Assays
Target:
PTX3
Synonyms:
TNFAIP5, TSG-14, Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta, Tumor Necrosis Factor Alpha-Induced Protein 5, Tumor necrosis factor-inducible gene 14 protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
