Human Peptidyl Arginine Deiminase Type II (PADI2) ELISA Kit
The Human Peptidyl Arginine Deiminase Type II (PADI2) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of PADI2 in human samples. This kit is specifically designed for the detection of PADI2 in serum, plasma, and cell culture supernatants with high sensitivity and specificity.PADI2 is an important enzyme involved in protein citrullination, a post-translational modification process that plays a role in various biological processes including gene regulation, immune response, and inflammation.
Dysregulation of PADI2 has been linked to several diseases such as autoimmune disorders, cancer, and neurodegenerative conditions.With this ELISA kit, researchers can obtain reliable and reproducible results, making it an invaluable tool for studying the role of PADI2 in disease pathology and for identifying potential therapeutic strategies. Invest in the Human PADI2 ELISA Kit to advance your research in the field of citrullination and its implications in human health.
Product Name:
Human Peptidyl Arginine Deiminase Type II (PADI2) ELISA Kit
Product Code:
HUDL02167
Size:
96 Assays
Target:
PADI2
Synonyms:
PAD-H19, PAD2, PDI2, Peptidylarginine deiminase II
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
