The Assay Genie Human PF4 (Platelet Factor 4) ELISA Kit is specifically developed to quantitatively measure the levels of Platelet Factor 4 (PF4) in a variety of biological samples. PF4, a chemokine protein released by platelets during the aggregation process, plays crucial roles in the regulation of blood coagulation, wound healing, and immune responses. Accurate quantification of PF4 is essential for understanding its functions in platelet function, vascular biology, and immunomodulation. Our PF4 ELISA Kit boasts exceptional sensitivity and specificity, ensuring precise and reproducible results for your research needs. Manufactured under strict quality control guidelines, this kit delivers robust performance and user-friendly protocols, making it an optimal choice for investigations focused on platelet biology, coagulation processes, and immune-related pathways. Trust in Assay Genie's PF4 ELISA Kit for dependable and accurate quantification of Platelet Factor 4 in your scientific endeavors.
Product Name:
Human PF4 (Platelet Factor 4) ELISA Kit
SKU:
AEES00184
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.375 ng/mL
Detection range:
0.63-40 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
