The Human PGF (Placental Growth Factor) ELISA Kit is specifically designed for the precise measurement of placental growth factor levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable tool for various research applications.PGF, also known as placental growth factor, is a key protein that plays a crucial role in angiogenesis and vascular development. Its involvement in conditions like cancer, cardiovascular diseases, and pregnancy-related complications highlight its importance as a biomarker for studying and potentially treating these conditions.With the Human PGF ELISA Kit, researchers can uncover valuable insights into PGF levels and its impact on various physiological processes, paving the way for advancements in diagnostics and personalized medicine.
Product Name:
Human PGF (Placental Growth Factor) ELISA Kit
SKU:
HUES02569
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.63 pg/mL
Detection range:
1.96-125 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
