Human PGP Recombinant Protein (RPPB2105)
- SKU:
- RPPB2105
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- A6NDG6
- Research Area:
- Enzymes
Description
| Product Name: | Human PGP Recombinant Protein |
| Product Code: | RPPB2105 |
| Size: | 10µg |
| Species: | Human |
| Target: | PGP |
| Synonyms: | Glycerol-3-phosphate phosphatase, G3PP, Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, AUM, Phosphoglycolate phosphatase, PGP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PGP protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAAAEA GGDDARCVRL SAERAQALLA DVDTLLFDCD GVLWRGETAV PGAPEALRAL RARGKRLGFI TNNSSKTRAA YAEKLRRLGF GGPAGPGASL EVFGTAYCTA LYLRQRLAGA PAPKAYVLGS PALAAELEAV GVASVGVGPE PLQGEGPGDW LHAPLEPDVR AVVVGFDPHF SYMKLTKALR YLQQPGCLLV GTNMDNRLPL ENGRFIAGTG CLVRAVEMAA QRQADIIGKP SRFIFDCVSQ EYGINPERTV MVGDRLDTDI LLGATCGLKT ILTLTGVSTL GDVKNNQESD CVSKKKMVPD FYVDSIADLL PALQG |
| Biological Activity: | Specific activity is > 3,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C. |
Phosphoglycolate phosphatase (PGP) is discovered in all tissues including red cells, lymphocytes and cultured fibroblasts (at protein level). PGP is most active in skeletal muscle and cardiac muscle. The catalytic activity of PGP is 2-phosphoglycolate + H2O = glycolate + phosphate. Diseases associated with PGP include tardive dyskinesia and polycystic kidney disease.
PGP Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 345 amino acids (1-321a.a) and having a molecular mass of 36.5kDa.PGP is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PGP: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.1.3.48; EC 3.1.3.18; Phosphatase (non-protein) Chromosomal Location of Human Ortholog: 16p13.3 Molecular Function:phosphoglycolate phosphatase activity; magnesium ion binding; protein tyrosine phosphatase activity Biological Process: dephosphorylation; carbohydrate metabolic process |
| UniProt Code: | A6NDG6 |
| NCBI GenInfo Identifier: | 166987826 |
| NCBI Gene ID: | 283871 |
| NCBI Accession: | A6NDG6.1 |
| UniProt Related Accession: | A6NDG6 |
| Molecular Weight: | 34,006 Da |
| NCBI Full Name: | Phosphoglycolate phosphatase |
| NCBI Synonym Full Names: | phosphoglycolate phosphatase |
| NCBI Official Symbol: | PGP�� |
| NCBI Protein Information: | phosphoglycolate phosphatase; PGPase |
| UniProt Protein Name: | Phosphoglycolate phosphatase |
| Protein Family: | Glycerol-3-phosphate phosphatase |
| UniProt Gene Name: | PGP�� |
| UniProt Entry Name: | PGP_HUMAN |
