Recombinant Human PDGF-AA Protein (His Tag)(Active) (RPES2146)

SKU:
RPES2146
€196

Description

ELISA Kit Technical Manual

Recombinant Human PDGF-AA Protein (His Tag)(Active)

Background:Platelet-derived growth factor subunit A (PDGFA), belongs to the PDGF/VEGF growth factor family. PDGFA is a secreted protein, stored in platelet alpha-granules and released by platelets upon wounding. PDGFA is potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. It plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. PDGFA is required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis, normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. It plays an important role in wound healing; Signaling is modulated by the formation of heterodimers with PDGFB.
Name:Recombinant Human PDGF-AA Protein (His Tag)(Active)
Size:10µg
Assay Genie SKU:RPES2146
Synonyms:Platelet-derived growth factor subunit A;PDGF subunit A;PDGF;Platelet-derived growth factor A chain;Platelet-derived growth factor alpha polypeptide; PDGFA;PDGF1
Species:Human
Expression host:E.coli
Sequence:Ser87-Thr 211
Accession:P04085
Mol Mass:15.9 kDa
AP Mol Mass:16 kDa
Tag:N-6His
Bio Activity:Measured in a cell proliferation assay using BALB/C?3T3 mouse fibroblast cells.  The ED50 for this effect is 100-400 ng/ml.
Purity:> 90 % as determined by reducing SDS-PAGE.
Endotoxin:< 1.0 EU per µg as determined by the LAL method.
Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8? for 2-7 days. Aliquots of reconstituted samples are stable at < -20'C for 3 months.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Formulation:Lyophilized from a 0.2 um filtered solution of PBS, pH7.4.
Reconstitution:Please refer to the printed manual for detailed information.
Application:Cell Culture
UniProt Protein Function:PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: Golgi membrane; extracellular space; microvillus; cell surface; endoplasmic reticulum lumen; extracellular region

Molecular Function:collagen binding; protein binding; protein homodimerization activity; growth factor activity; protein heterodimerization activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding

Biological Process: skin development; extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; wound healing; negative chemotaxis; platelet-derived growth factor receptor signaling pathway; cell projection biogenesis; response to estradiol stimulus; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; regulation of smooth muscle cell migration; platelet degranulation; hair follicle development; positive regulation of MAPKKK cascade; cell-cell signaling; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to wounding; angiogenesis; inner ear development; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; response to drug; platelet activation; response to inorganic substance; phosphoinositide-mediated signaling; response to retinoic acid; fibroblast growth factor receptor signaling pathway; negative regulation of phosphatidylinositol biosynthetic process; positive regulation of protein amino acid autophosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; cell activation; organ morphogenesis; regulation of actin cytoskeleton organization and biogenesis; positive regulation of cell division; response to hypoxia; innate immune response; actin cytoskeleton organization and biogenesis; blood coagulation; alveolus development; positive regulation of DNA replication; positive regulation of cell migration

NCBI Summary:This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
UniProt Code:P04085
NCBI GenInfo Identifier:77695917
NCBI Gene ID:5154
NCBI Accession:NP_002598
UniProt Secondary Accession:P04085,B5BU73,
UniProt Related Accession:P04085
Molecular Weight:22,253 Da
NCBI Full Name:platelet-derived growth factor subunit A isoform 1 preproprotein
NCBI Synonym Full Names:platelet derived growth factor subunit A
NCBI Official Symbol:PDGFA  
NCBI Official Synonym Symbols:PDGF1; PDGF-A  
NCBI Protein Information:platelet-derived growth factor subunit A
UniProt Protein Name:Platelet-derived growth factor subunit A
UniProt Synonym Protein Names:PDGF; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide
Protein Family:Platelet-derived growth factor
UniProt Gene Name:PDGFA  
UniProt Entry Name:PDGFA_HUMAN
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Additional Information

Product type:
Recombinant Protein
Host species:
E.coli
Reactivity:
Human
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