Description
Human PHF1 Recombinant Protein (N-His)
Human PHF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Leu31-Arg334, with an N-terminal His tag. Predicted molecular weight: 36.90 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. PHD finger protein 1 is a ~62 kDa protein. Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. For research use only.
Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2.
| Product Name: | Human PHF1 Recombinant Protein (N-His) |
| SKU: | GXRP11175 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PCL1, PHD finger protein 1, PHF1, Polycomb-like protein 1, Protein PHF1, hPCl1, hPHF1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Leu31-Arg334 |
| Calculated MW: | 36.90 kDa |
| UniProt: | O43189 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
