Human Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit
The Human Phosphoglycerate Mutase 1 (PGAM1) Brain ELISA Kit is a powerful tool for detecting and quantifying PGAM1 levels in human brain tissue samples. This kit is designed with high sensitivity and specificity, providing accurate and reliable results for research applications.PGAM1 is an essential enzyme involved in glycolysis and energy metabolism in the brain. Dysregulation of PGAM1 has been linked to various neurological disorders such as Alzheimer's disease, Parkinson's disease, and epilepsy.
Therefore, measuring PGAM1 levels in brain tissue can provide valuable insights into the pathogenesis of these conditions and potential therapeutic targets.With the Human PGAM1 Brain ELISA Kit, researchers can advance their understanding of the role of PGAM1 in neurological disorders and pave the way for the development of novel diagnostic and therapeutic strategies. Order your kit today and unlock the secrets of PGAM1 in the brain.
Product Name:
Human Phosphoglycerate Mutase 1, Brain (PGAM1) ELISA Kit
Product Code:
HUDL02270
Size:
96 Assays
Target:
PGAM1
Synonyms:
PGAMA, PGAM-B, BPG-dependent PGAM 1, Phosphoglycerate mutase isozyme B
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
