Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit
The Human Phospholipase A2 Group IVD (PLA2G4D) ELISA Kit is a cutting-edge tool designed for the precise measurement of PLA2G4D levels in human biological samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research applications.PLA2G4D is a key enzyme belonging to the phospholipase A2 family, playing a crucial role in lipid metabolism and inflammation regulation. Dysregulation of PLA2G4D has been implicated in various pathological conditions, including inflammatory diseases, neurodegenerative disorders, and cancer.
Therefore, monitoring PLA2G4D levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With the Human Phospholipase A2 Group IVD ELISA Kit, researchers can uncover the intricate roles of PLA2G4D in health and disease, paving the way for innovative discoveries and advancements in biomedicine. Trust in this advanced kit to accelerate your research efforts and deepen your understanding of PLA2G4D biology.
Product Name:
Human Phospholipase A2, Group IVD (PLA2G4D) ELISA Kit
Product Code:
HUDL02321
Size:
96 Assays
Target:
PLA2G4D
Synonyms:
cPLA2delta, Cytosolic phospholipase A2 delta
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
91-99
95
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
