Human Pituitary adenylate cyclase-activating polypeptide/PACAP ELISA Kit
The Human Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) ELISA Kit is a powerful tool for detecting PACAP levels in human biological samples. This kit offers high sensitivity and specificity, enabling researchers to obtain accurate and reproducible results in serum, plasma, and cell culture supernatants. PACAP is a neuropeptide that plays a critical role in various physiological processes, including hormone secretion, neurotransmission, and cell proliferation. Dysregulation of PACAP signaling has been linked to neurological disorders, stress responses, and inflammatory conditions, making it a valuable target for research in these areas.
With the Human PACAP ELISA Kit, researchers can delve into the intricate mechanisms of PACAP function and its potential therapeutic applications in the fields of neuroscience, endocrinology, and immunology. Trust in the precision and reliability of this kit to advance your understanding of PACAP biology and its implications for human health.
Product Name:
Human Pituitary adenylate cyclase-activating polypeptide/PACAP ELISA Kit
SKU:
HUFI01234
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
89-105%
93-104%
92-99%
EDTA Plasma (n = 5)
89-98%
82-100%
88-100%
Heparin Plasma (n = 5)
80-100%
81-97%
81-93%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-101
95
EDTA Plasma (n = 5)
89-104
98
Heparin Plasma (n = 5)
88-104
99
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Concentrated, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
HRP-Streptavidin Conjugate (SABC, 100X)
60 ul
120 ul
2-8°C (Avoid direct light)
TMB Substrate
5 ml
10 ml
2-8°C (Avoid direct light)
Sample Dilution Buffer
10 ml
20 ml
2-8°C
Antibody Dilution Buffer
5 ml
10 ml
2-8°C
SABC Dilution Buffer
5 ml
10 ml
2-8°C
Stop Solution
5 ml
10 ml
2-8°C
Wash Buffer(25X)
15 ml
30 ml
2-8°C
Plate Sealer
3 pieces
5 pieces
-
Technical Manual
1 copy
1 copy
-
Sun et al.
Decreased synovial fluid pituitary adenylate cyclase-activating polypeptide (PACAP) levels may reflect disease severity in post-traumatic knee osteoarthritis after anterior cruciate ligament injury
The synovial fluid neuropeptide PACAP may act as a protective factor during disease progression of primary knee osteoarthritis and is increased following hyaluronic acid injection
Correlation of Decreased Serum Pituitary Adenylate Cyclase-Activating Polypeptide and Vasoactive Intestinal Peptide Levels With Non-motor Symptoms in Patients With Parkinson's Disease
