The Human Prokineticin-2 (PK2) ELISA Kit is a reliable and accurate tool for detecting prokineticin-2 levels in human samples such as serum, plasma, and cell culture supernatants. Prokineticin-2 is a key protein that plays a role in various biological processes including angiogenesis, inflammation, and pain perception.This ELISA kit offers high sensitivity and specificity, providing researchers with dependable and precise results for their studies. Prokineticin-2 is known to be involved in several health conditions such as cancer, cardiovascular diseases, and neurological disorders, making it a valuable biomarker for research and potential therapeutic development.
With easy-to-follow instructions and a user-friendly format, the Human Prokineticin-2 (PK2) ELISA Kit is an essential tool for investigating the functions and implications of prokineticin-2 in human health and disease. Choose this kit for reliable and reproducible results in your research applications.
Product Name:
Human Prokineticin 2 (PK2) ELISA Kit
Product Code:
HUDL02299
Size:
96 Assays
Target:
PK2
Synonyms:
PK-2, Bv8, MIT1, KAL4, Protein Bv8 Homolog
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
