Description
Human PLA2G6 Recombinant Protein (N-His)
Human PLA2G6 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp484-Val701, with an N-terminal His tag. Predicted molecular weight: 26.51 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. 85/88 kDa calcium-independent phospholipase A2 (PLA2G6) is a ~89 kDa protein. Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. For research use only.
Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content.
| Product Name: | Human PLA2G6 Recombinant Protein (N-His) |
| SKU: | GXRP03354 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Patatin-like phospholipase domain-containing protein 9, Group VI phospholipase A2, iPLA2-beta, PLA2G6, PLPLA9, Intracellular membrane-associated calcium-independent phospholipase A2 beta, GVI PLA2, CaI-PLA2, Palmitoyl-CoA hydrolase, 2-lysophosphatidylcholine acylhydrolase, 85/88 kDa calcium-independent phospholipase A2, PNPLA9 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp484-Val701 |
| Calculated MW: | 26.51 kDa |
| UniProt: | O60733 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
