Western blot analysis of Human plasma, using Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (CAB24499) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features
Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody (CAB24499)
Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody (CAB24499)
The Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody (CAB24499) is a high-quality antibody developed for reliable detection and analysis of target proteins. This enzyme plays a crucial role in lipid metabolism and inflammation regulation. The antibody, derived from rabbit sources, exhibits high specificity and sensitivity for human samples, making it an ideal choice for Western blot applications.By targeting the PLA2G7 protein, this antibody enables precise detection and analysis in various cell types, providing valuable insights into lipid signaling pathways and inflammatory processes.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Human PLA2G7/PAF-AH/Lp-PLA2 Polyclonal Antibody
SKU:
CAB24499
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PLA2G7, LDL-PLA2, LP-PLA2, PAFAD, PAFAH, phospholipase A2 group VII, Human PLA2G7/PAF-AH/Lp-PLA2
Positive Sample:
Human plasma
Cellular Localization:
Secreted, Extracellular Space.
Calculated MW:
50kDa
Observed MW:
60kDa
The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
Purification Method
Affinity purification
Gene ID
7941
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Human plasma, using Human PLA2G7/PAF-AH/Lp-PLA2 Rabbit pAb (CAB24499) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
