Human PLK1/Serine/threonine- kinase PLK1 ELISA Kit
The Human PLK1 (Serine/Threonine Kinase PLK1) ELISA Kit is specifically designed for the precise measurement of PLK1 levels in human samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal tool for various research applications. PLK1, also known as Polo-like kinase 1, is a key regulator of cell cycle progression and is involved in various cellular processes including mitosis and DNA damage response. Dysregulation of PLK1 has been linked to cancer development and progression, highlighting its significance as a potential therapeutic target.
By detecting and quantifying PLK1 levels, researchers can gain valuable insights into its role in disease pathogenesis and identify novel treatment strategies. Overall, the Human PLK1 ELISA Kit offers a reliable and efficient method for the measurement of PLK1 levels, enabling researchers to advance their understanding of this important protein and its implications in disease biology.
Product Name:
Human PLK1/Serine/threonine- kinase PLK1 ELISA Kit
SKU:
HUFI01566
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
4.688 pg/mL
Range:
7.813-500 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
82-100%
87-103%
90-98%
EDTA Plasma (n = 5)
82-98%
82-98%
85-105%
Heparin Plasma (n = 5)
82-101%
87-101%
82-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
87-101
94
EDTA Plasma (n = 5)
87-100
96
Heparin Plasma (n = 5)
94-99
97
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
