Description
Human PMS2 Recombinant Protein (N-His)
Human PMS2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asp19-Asn371, with an N-terminal His tag. Predicted molecular weight: 41.51 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Mismatch repair endonuclease PMS2 is a ~95 kDa protein. Component of the post-replicative DNA mismatch repair system (MMR). For research use only.
Heterodimerizes with MLH1 to form MutL alpha. DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.
| Product Name: | Human PMS2 Recombinant Protein (N-His) |
| SKU: | GXRP09117 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Mismatch repair endonuclease PMS2; 3.1.-.-; DNA mismatch repair protein PMS2; PMS1 protein homolog 2; PMS2; PMSL2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asp19-Asn371 |
| Calculated MW: | 41.51 kDa |
| UniProt: | P54278 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
