Description
Human POLB Recombinant Protein (N-His)
Human POLB Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn12-Glu335, with an N-terminal His tag. Predicted molecular weight: 39.20 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA polymerase beta (POLB) is a ~38 kDa protein. Repair polymerase that plays a key role in base-excision repair. For research use only.
During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.
| Product Name: | Human POLB Recombinant Protein (N-His) |
| SKU: | GXRP05004 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA polymerase beta, POLB |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn12-Glu335 |
| Calculated MW: | 39.20 kDa |
| UniProt: | P06746 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
