Description
Human POLI Recombinant Protein (N-His)
Human POLI Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Arg53-Lys439, with an N-terminal His tag. Predicted molecular weight: 46.03 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA polymerase iota (POLI) is a ~83 kDa protein. Error-prone DNA polymerase specifically involved in DNA repair. For research use only.
Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.
| Product Name: | Human POLI Recombinant Protein (N-His) |
| SKU: | GXRP04497 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RAD30 homolog B, RAD30B, DNA polymerase iota, Eta2, POLI |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Arg53-Lys439 |
| Calculated MW: | 46.03 kDa |
| UniProt: | Q9UNA4 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
