Description
Human POLR2B Recombinant Protein (N-His)
Human POLR2B Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Val40-Leu395, with an N-terminal His tag. Predicted molecular weight: 42.85 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. DNA-directed RNA polymerase II subunit RPB2 is a ~133 kDa protein. Catalytic core component of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase which synthesizes mRNA precursors and many functional non-coding RNAs using the four ribonucleoside triphosphates as substrates. For research use only.
Pol II-mediated transcription cycle proceeds through transcription initiation, transcription elongation and transcription termination stages. During transcription initiation, Pol II pre-initiation complex (PIC) is recruited to DNA promoters, with focused-type promoters containing either the initiator (Inr) element, or the TATA-box found in cell-type specific genes and dispersed-type promoters that often contain hypomethylated CpG islands usually found in housekeeping genes. Once the polymerase has escaped from the promoter it enters the elongation phase during which RNA is actively polymerized, based on complementarity with the template DNA strand. Transcription termination involves the release of the RNA transcript and polymerase from the DNA.
| Product Name: | Human POLR2B Recombinant Protein (N-His) |
| SKU: | GXRP09140 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | DNA-directed RNA polymerase II subunit RPB2; 2.7.7.6; 3'-5' exoribonuclease; 3.1.13.-; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2; 2.7.7.48; POLR2B |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Val40-Leu395 |
| Calculated MW: | 42.85 kDa |
| UniProt: | P30876 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only |
