Human Poly A Specific Ribonuclease (PARN) ELISA Kit
The Human Poly(A)-specific Ribonuclease (PARN) ELISA Kit is specially designed for the precise measurement of PARN levels in human serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers dependable and consistent results, catering to a variety of research requirements.PARN is an important enzyme involved in RNA decay processes, specifically targeting poly(A) tails on mRNA molecules. Dysregulation of PARN has been linked to various diseases, including cancer, metabolic disorders, and neurological conditions, underscoring its significance as a biomarker for disease progression and potential therapeutic interventions.
Explore the intricate mechanisms of RNA decay and its implications in disease pathology with the Human PARN ELISA Kit, a valuable tool for advancing biomedical research and uncovering novel insights into molecular pathways.
Product Name:
Human Poly A Specific Ribonuclease (PARN) ELISA Kit
Product Code:
HUDL02192
Size:
96 Assays
Target:
PARN
Synonyms:
DAN, Deadenylation Nuclease, Deadenylating nuclease, Polyadenylate-specific ribonuclease
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
