Description
Human PPIF Recombinant Protein (N-His)
Human PPIF Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Asn45-Lys196, with an N-terminal His tag. Predicted molecular weight: 18.72 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Peptidyl-prolyl cis-trans isomerase F, mitochondrial (PPIF) is a ~22 kDa protein. PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. For research use only.
Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial p53/TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F(1)F(0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels.
| Product Name: | Human PPIF Recombinant Protein (N-His) |
| SKU: | GXRP01399 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | CypD, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, Cyclophilin D, Cyclophilin F, CYP3, CyP-M, Rotamase F, PPIF, CyP-D, PPIase F, Mitochondrial cyclophilin |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Asn45-Lys196 |
| Calculated MW: | 18.72 kDa |
| UniProt: | P30405 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
