Description
Human PPM1K Recombinant Protein (N-His)
Human PPM1K Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Ser91-Ala372, with an N-terminal His tag. Predicted molecular weight: 33.29 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Protein phosphatase Mn(2+)-dependent 1K (PPM1K) is a ~40 kDa protein. Serine/threonine-protein phosphatase component of macronutrients metabolism. For research use only.
Forms a functional kinase and phosphatase pair with BCKDK, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. At high levels of branched-chain ketoacids, dephosphorylates and activates mitochondrial BCKDH complex, a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Tightly associates with the E2 component of BCKDH complex and dephosphorylates BCKDHA on Ser-337. Regulates the reversible phosphorylation of ACLY in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition.
| Product Name: | Human PPM1K Recombinant Protein (N-His) |
| SKU: | GXRP04781 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PP2C-type mitochondrial phosphoprotein phosphatase, PP2C-like mitochondrial protein, Protein phosphatase 1K, mitochondrial, PP2CM, PP2C-kappa, PPM1K, PTMP, Protein phosphatase 2C isoform kappa, PP2C domain-containing protein phosphatase 1K |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Ser91-Ala372 |
| Calculated MW: | 33.29 kDa |
| UniProt: | Q8N3J5 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
