The Human PR3 (Proteinase 3) ELISA Kit is specifically designed for the precise detection of PR3 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results that are suitable for a variety of research purposes.PR3, also known as Proteinase 3, is a critical enzyme involved in immune responses and inflammatory processes. Its dysregulation has been linked to various autoimmune diseases, such as vasculitis and rheumatoid arthritis, highlighting its importance as a biomarker for studying these conditions and exploring potential treatment options. Overall, the Human PR3 ELISA Kit provides a valuable tool for researchers looking to investigate the role of PR3 in human health and disease, offering a reliable method for quantifying PR3 levels in biological samples.
Product Name:
Human PR3 (Proteinase 3) ELISA Kit
SKU:
HUES02909
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
