Description
Human PRDM9 Recombinant Protein (N-His)
Human PRDM9 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Gln198-Gly380, with an N-terminal His tag. Predicted molecular weight: 23.15 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Histone-lysine N-methyltransferase PRDM9 is a ~103 kDa protein. Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. For research use only.
Can also methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression.
| Product Name: | Human PRDM9 Recombinant Protein (N-His) |
| SKU: | GXRP07710 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Histone-lysine N-methyltransferase PRDM9, PR domain zinc finger protein 9, PR domain-containing protein 9, Protein-lysine N-methyltransferase PRDM9, 2.1.1.-, [histone H3]-lysine36 N-trimethyltransferase PRDM9, 2.1.1.359, [histone H3]-lysine4 N-trimethyltransferase PRDM9, 2.1.1.354, [histone H3]-lysine9 N-trimethyltransferase PRDM9, 2.1.1.355, [histone H4]-N-methyl-L-lysine20 N-methyltransferase PRDM9, 2.1.1.362, [histone H4]-lysine20 N-methyltransferase PRDM9, 2.1.1.361, PRDM9, PFM6 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Gln198-Gly380 |
| Calculated MW: | 23.15 kDa |
| UniProt: | Q9NQV7 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
