The Human PRL (Prolactin) ELISA Kit is specifically designed for the precise measurement of prolactin levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for various research applications.Prolactin is a hormone that plays a crucial role in lactation, reproductive health, and immune function. Abnormal levels of prolactin have been associated with conditions such as infertility, menstrual irregularities, and pituitary tumors, making it a valuable biomarker for studying these disorders and developing potential treatments. With the Human PRL ELISA Kit, researchers can reliably and efficiently analyze prolactin levels in biological samples, providing valuable insights into the role of this hormone in health and disease.
Product Name:
Human PRL (Prolactin) ELISA Kit
SKU:
HUES01416
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
