SynonymsPRO-C,VKDPC,PC,Vitamin K Dependent Protein C,Inactivator Of Coagulation Factors Va And VIIIa,Autoprothrombin IIA,Blood coagulation factor XIV,Activation peptide
ReactivityHuman
Sample TypePlasma
SKU:
HUDL02409
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04070
Sensitivity:
0.54ng/mL
Range:
1.25-80ng/mL
ELISA Type:
Sandwich
Synonyms:
PRO-C,VKDPC,PC,Vitamin K Dependent Protein C,Inactivator Of Coagulation Factors Va And VIIIa,Autoprothrombin IIA,Blood coagulation factor XIV,Activation peptide
The Human C-PROC (Procalcitonin) ELISA Kit is specifically designed for the quantitative measurement of procalcitonin levels in human serum, plasma, and tissue homogenates. This kit offers superior sensitivity and accuracy, providing researchers with reliable and consistent results for their studies.Procalcitonin is a precursor protein to the hormone calcitonin and is a key biomarker for sepsis and other inflammatory conditions. Elevated procalcitonin levels are often seen in patients with bacterial infections, making it a valuable tool for early diagnosis and monitoring of sepsis progression.
Suitable for a wide range of research applications, the Human C-PROC ELISA Kit is a must-have tool for researchers studying sepsis, inflammation, and infectious diseases. With its high performance and ease of use, this kit is essential for any laboratory looking to advance their research in these critical areas of study.
Product Name:
Human Protein C (PROC) ELISA Kit
Product Code:
HUDL02409
Size:
96 Assays
Target:
PROC
Synonyms:
PRO-C, VKDPC, PC, Vitamin K Dependent Protein C, Inactivator Of Coagulation Factors Va And VIIIa, Autoprothrombin IIA, Blood coagulation factor XIV, Activation peptide
Detection Method:
Sandwich
Range:
1.25-80ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
