Human Programmed Cell Death Protein 6 (PDCD6) ELISA Kit
The Human Programmed Cell Death Protein 6 (PDCD6) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PDCD6 levels in human samples such as serum, plasma, and cell culture supernatants. PDCD6 is a protein involved in programmed cell death, also known as apoptosis, and plays a critical role in various cellular processes such as cell survival, proliferation, and development. Dysregulation of PDCD6 has been linked to a variety of diseases including cancer, autoimmune disorders, and neurodegenerative diseases, highlighting its importance as a potential biomarker in research and clinical applications.
With its reliable and reproducible results, the Human PDCD6 ELISA Kit is an essential tool for studying the role of PDCD6 in various biological pathways and diseases, as well as for developing new therapeutic strategies targeting this protein.
Product Name:
Human Programmed Cell Death Protein 6 (PDCD6) ELISA Kit
Product Code:
HUDL02223
Size:
96 Assays
Target:
PDCD6
Synonyms:
ALG-2, PEF1B, Apoptosis-Linked Gene-2, Probable calcium-binding protein ALG-2
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
