Human Protein C Receptor, Endothelial (PROCR) ELISA Kit
The Human Protein C Receptor (ProCR) ELISA Kit is specifically designed for the precise quantification of ProCR levels in human samples. Whether testing serum, plasma, or cell culture supernatants, this kit delivers consistent and accurate results with high sensitivity and specificity.ProCR is a vital receptor involved in regulating protein C activation, playing a key role in maintaining hemostasis and preventing excessive blood clotting.
Dysregulation of ProCR has been implicated in various disease states, including thrombosis, sepsis, and inflammatory conditions, making it a valuable biomarker for studying these pathologies and developing targeted therapies.Trust the Human Protein C Receptor ELISA Kit to provide reliable data for your research needs, enabling advanced investigations into ProCR function and its potential implications in disease mechanisms.
Product Name:
Human Protein C Receptor, Endothelial (PROCR) ELISA Kit
Product Code:
HUDL02410
Size:
96 Assays
Target:
PROCR
Synonyms:
CD201, CCCA, CCD41, BA42O4.2, EPCR, Endothelial Protein C Receptor, Cell Cycle, Centrosome-Associated Protein, Activated Protein C Receptor
Detection Method:
Sandwich
Range:
0.625-40ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
