Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit
The Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PDIA5 levels in human samples such as serum, plasma, and cell culture supernatants. PDIA5 is a crucial protein involved in maintaining protein folding and redox homeostasis within cells, making it a key player in cellular functions and disease processes.This ELISA kit offers researchers a reliable and reproducible method for quantifying PDIA5 levels, allowing for precise measurements and comparisons across different samples.
By studying PDIA5 levels, researchers can gain valuable insights into its roles in various diseases and biological processes, as well as its potential as a therapeutic target.Overall, the Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit is a valuable tool for researchers looking to explore the functions and implications of PDIA5 in human health and disease.
Product Name:
Human Protein Disulfide Isomerase A5 (PDIA5) ELISA Kit
Product Code:
HUDL02248
Size:
96 Assays
Target:
PDIA5
Synonyms:
PDI-A5, PDIR, Protein Disulfide Isomerase-Associated 5, Protein disulfide isomerase-related protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
