Human Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B) ELISA Kit
The Human Protein Phosphatase 1 Regulatory Subunit 1B (PPP1R1B) ELISA Kit is specifically designed for the accurate measurement of PPP1R1B levels in human biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research purposes.PPP1R1B is a critical regulatory subunit of protein phosphatase 1, playing a key role in various cellular processes, including cell signaling, metabolism, and gene expression.
Dysregulation of PPP1R1B has been implicated in several diseases such as cancer, neurodegenerative disorders, and cardiovascular diseases, highlighting its importance as a biomarker for disease diagnosis and therapeutic development.Overall, the Human Protein Phosphatase 1 Regulatory Subunit 1B (PPP1R1B) ELISA Kit is a valuable tool for researchers studying the role of PPP1R1B in health and disease, providing reliable and accurate measurement of PPP1R1B levels in human samples.
Product Name:
Human Protein Phosphatase 1, Regulatory Subunit 1B (PPP1R1B) ELISA Kit
Product Code:
HUDL02380
Size:
96 Assays
Target:
PPP1R1B
Synonyms:
DARPP-32, Dopamine And cAMP Regulated Phosphoprotein, Dopamine and cAMP-regulated neuronal phosphoprotein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-98
94
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
