Human Protein Tyrosine Phosphatase,Non Receptor Type 1 (PTPN1) ELISA Kit
The Human Protein Tyrosine Phosphatase Non-Receptor Type 1 (PTPN1) ELISA Kit from Assay Genie is designed for the precise measurement of PTPN1 levels in human biological samples. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications. PTPN1 is a key enzyme involved in regulating cell signaling pathways by dephosphorylating target proteins. It plays a critical role in cellular processes such as cell growth, differentiation, and immune response. Dysregulation of PTPN1 has been linked to various diseases, including cancer, diabetes, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.
Researchers can rely on the Human PTPN1 ELISA Kit to provide precise and reliable measurements of PTPN1 levels in a convenient and efficient format. This kit is an essential tool for advancing research in cell signaling, disease biology, and drug discovery. Order your Human PTPN1 ELISA Kit from Assay Genie today to accelerate your research and uncover new insights into the role of PTPN1 in human health and disease.
Product Name:
Human Protein Tyrosine Phosphatase,Non Receptor Type 1 (PTPN1) ELISA Kit
Product Code:
HUDL02459
Size:
96 Assays
Target:
PTPN1
Synonyms:
PTP1B, PTP1-B, Protein-tyrosine phosphatase 1B
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
