Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit
The Human Protein Tyrosine Phosphatase Non-Receptor Type 11 (PTPN11) ELISA Kit is specifically designed for the accurate detection of PTPN11 levels in human serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PTPN11, also known as SHP2, is an important protein involved in signal transduction pathways and cell growth regulation. Dysregulation of PTPN11 has been linked to various diseases including cancer, developmental disorders, and metabolic conditions, making it a valuable biomarker for studying these conditions and potentially developing targeted therapies.
With easy-to-follow protocols and a user-friendly format, the Human PTPN11 ELISA Kit is an essential tool for researchers looking to accurately quantify PTPN11 levels in biological samples and further understand its role in disease pathogenesis.
Product Name:
Human Protein Tyrosine Phosphatase, Non Receptor Type 11 (PTPN11) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
