Human Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) ELISA Kit
The Human Proteolipid Protein 2 (PLP2) ELISA Kit is a valuable tool for the precise measurement of PLP2 levels in colonic epithelium-enriched samples. This kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.PLP2 is an important protein found in the colonic epithelium, playing a key role in maintaining intestinal barrier function and regulating immune responses.
Dysregulation of PLP2 expression has been linked to inflammatory bowel diseases and other gastrointestinal conditions, making it a valuable biomarker for studying these disorders and developing potential treatments.With the Human PLP2 ELISA Kit, researchers can explore the role of PLP2 in colonic epithelium function and pathology, ultimately advancing our understanding of gastrointestinal health and disease.
Product Name:
Human Proteolipid Protein 2, Colonic Epithelium Enriched (PLP2) ELISA Kit
Product Code:
HUDL02346
Size:
96 Assays
Target:
PLP2
Synonyms:
A4, A4-LSB, A4 Differentiation-Dependent Protein, Intestinal membrane A4 protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
