Description
Human PRPF8 Recombinant Protein (N-His)
Human PRPF8 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Thr2071-Ala2335, with an N-terminal His tag. Predicted molecular weight: 32.80 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Pre-mRNA-processing-splicing factor 8 (PRPF8) is a ~273 kDa protein. Plays a role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. For research use only.
Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as a scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.
| Product Name: | Human PRPF8 Recombinant Protein (N-His) |
| SKU: | GXRP04874 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | 220 kDa U5 snRNP-specific protein, p220, PRPF8, Splicing factor Prp8, PRP8 homolog, Pre-mRNA-processing-splicing factor 8, PRPC8 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Thr2071-Ala2335 |
| Calculated MW: | 32.80 kDa |
| UniProt: | Q6P2Q9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
