Human PSMB3 Recombinant Protein (RPPB2206)
- SKU:
- RPPB2206
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49720
- Research Area:
- Enzymes
Description
| Product Name: | Human PSMB3 Recombinant Protein |
| Product Code: | RPPB2206 |
| Size: | 20µg |
| Species: | Human |
| Target: | PSMB3 |
| Synonyms: | Proteasome (prosome, macropain) subunit beta type 3, Proteasome chain 13, HC10-II, Proteasome component C10-II, Proteasome theta chain, EC 3.4.25.1, MGC4147. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | PSMB3 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 1mM DTT and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD |
The proteasome is a multicatalytic proteinase complex with an extremely organized ring-shaped 20S core structure. The core structure consists of 4 rings of 28 non-identical subunits; 2 rings consist of 7 alpha subunits and 2 rings consist of 7 beta subunits. Proteasomes are spread all over the eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent procedure in a non-lysosomal pathway. A vital role of a modified proteasome, the immunoproteasome, is the handling of class I MHC peptides.
PSMB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-205 a.a.) and having a molecular mass of 25.1kDa.PSMB3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMB3: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1B family. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; EC 3.4.25.1; Protease; Proteasome complex Chromosomal Location of Human Ortholog: 17q12 Cellular Component: cytosol; nucleoplasm; nucleus; proteasome complex; proteasome core complex Molecular Function:protein binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein deubiquitination; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; transmembrane transport; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013] |
| UniProt Code: | P49720 |
| NCBI GenInfo Identifier: | 20532411 |
| NCBI Gene ID: | 5691 |
| NCBI Accession: | P49720.2 |
| UniProt Secondary Accession: | P49720,P31147, Q0P6J7, Q96E27, |
| UniProt Related Accession: | P49720 |
| Molecular Weight: | 23kDa |
| NCBI Full Name: | Proteasome subunit beta type-3 |
| NCBI Synonym Full Names: | proteasome subunit beta 3 |
| NCBI Official Symbol: | PSMB3�� |
| NCBI Official Synonym Symbols: | HC10-II�� |
| NCBI Protein Information: | proteasome subunit beta type-3 |
| UniProt Protein Name: | Proteasome subunit beta type-3 |
| UniProt Synonym Protein Names: | Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PSMB3�� |
