Human PSMB5 Recombinant Protein (RPPB2208)
- SKU:
- RPPB2208
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P28074
- Research Area:
- Enzymes
Description
| Product Name: | Human PSMB5 Recombinant Protein |
| Product Code: | RPPB2208 |
| Size: | 10µg |
| Species: | Human |
| Target: | PSMB5 |
| Synonyms: | Proteasome subunit beta type-5, Macropain epsilon chain, Multicatalytic endopeptidase complex epsilon chain, Proteasome chain 6, Proteasome epsilon chain, Proteasome subunit MB1, Proteasome subunit X, PSMB5, LMPX, MB1, X, MGC104214. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PSMB5 solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P |
PSMB5 belongs to the proteasome B-type family that has a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is substituted by catalytic subunit 3i (proteasome beta 8 subunit). A fundamental function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB5 exhibits an ATP dependent proteolytic activity and is involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.
PSMB5 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 241 amino acids (60-263 a.a.) and having a molecular mass of 26.7kDa. The PSMB5 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.25.1; Proteasome complex Chromosomal Location of Human Ortholog: 14q11.2 Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol Molecular Function:threonine endopeptidase activity; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle |
| NCBI Summary: | The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009] |
| UniProt Code: | P28074 |
| NCBI GenInfo Identifier: | 187608890 |
| NCBI Gene ID: | 5693 |
| NCBI Accession: | P28074.3 |
| UniProt Secondary Accession: | P28074,Q16242, Q6PEW2, Q7Z3B5, Q86T01, Q9TNN9, B2R4N9 B4DUM9, D3DS43, E9PAV2, |
| UniProt Related Accession: | P28074 |
| Molecular Weight: | Calculated MW: 17kDa, 21kDa, 28kDaObserved MW: 21kDa |
| NCBI Full Name: | Proteasome subunit beta type-5 |
| NCBI Synonym Full Names: | proteasome (prosome, macropain) subunit, beta type, 5 |
| NCBI Official Symbol: | PSMB5�� |
| NCBI Official Synonym Symbols: | X; MB1; LMPX�� |
| NCBI Protein Information: | proteasome subunit beta type-5; proteasome chain 6; proteasome subunit X; proteasome subunit MB1; macropain epsilon chain; proteasome epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome subunit, beta type, 5; PSX large multifunctional protease X; multicatalytic endopeptidase complex epsilon chain |
| UniProt Protein Name: | Proteasome subunit beta type-5 |
| UniProt Synonym Protein Names: | Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X |
| Protein Family: | Proteasome |
| UniProt Gene Name: | PSMB5�� |
| UniProt Entry Name: | PSB5_HUMAN |
