Human PSMD9 Recombinant Protein (RPPB2215)
- SKU:
- RPPB2215
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O00233
- Research Area:
- Enzymes
Description
| Product Name: | Human PSMD9 Recombinant Protein |
| Product Code: | RPPB2215 |
| Size: | 20µg |
| Species: | Human |
| Target: | PSMD9 |
| Synonyms: | p27, Rpn4, 26S proteasome non-ATPase regulatory subunit 9, 26S proteasome regulatory subunit p27, PSMD9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The PSMD9 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR |
PSMD9 is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. PSMD9 acts as a chaperone through the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex compilation, PSMD9 is part of an intermediate PSMD9:PSMC6:PSMC3 module, also called modulator trimer complex. PSMD9 is released during the further base assembly process.
PSMD9 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-223) and having a molecular mass of 27.1kDa. PSMD9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PSMD9: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Belongs to the proteasome subunit p27 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Protease; Proteasome complex Chromosomal Location of Human Ortholog: 12q24.31-q24.32 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:bHLH transcription factor binding; protein binding; transcription coactivator activity Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; MAPKKK cascade; negative regulation of insulin secretion; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of insulin secretion; positive regulation of transcription, DNA-dependent; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein polyubiquitination; regulation of amino acid metabolic process; regulation of mRNA stability; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway |
| NCBI Summary: | The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | O00233 |
| NCBI GenInfo Identifier: | 317373521 |
| NCBI Gene ID: | 5715 |
| NCBI Accession: | O00233.3 |
| UniProt Secondary Accession: | O00233,Q9BQ42, B2RD35, G3V1Q6, |
| UniProt Related Accession: | O00233 |
| Molecular Weight: | 13,053 Da |
| NCBI Full Name: | 26S proteasome non-ATPase regulatory subunit 9 |
| NCBI Synonym Full Names: | proteasome 26S subunit, non-ATPase 9 |
| NCBI Official Symbol: | PSMD9�� |
| NCBI Official Synonym Symbols: | p27; Rpn4�� |
| NCBI Protein Information: | 26S proteasome non-ATPase regulatory subunit 9 |
| UniProt Protein Name: | 26S proteasome non-ATPase regulatory subunit 9 |
| UniProt Synonym Protein Names: | 26S proteasome regulatory subunit p27 |
| Protein Family: | 26S proteasome non-ATPase regulatory |
| UniProt Gene Name: | PSMD9�� |
| UniProt Entry Name: | PSMD9_HUMAN |
