Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
The Human PTGS2 (COX-2) Prostaglandin-Endoperoxide Synthase 2 ELISA Kit is a powerful tool for accurate quantification of PTGS2 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring trustworthy and reproducible results for a variety of research applications.PTGS2, also known as COX-2, is an enzyme crucial in the biosynthesis of prostaglandins, playing a vital role in inflammation and pain responses. Elevated levels of PTGS2 have been linked to various diseases like cancer, cardiovascular issues, and inflammatory disorders. Therefore, the Human PTGS2 ELISA Kit serves as a valuable resource for investigating the role of this enzyme in disease pathogenesis and potential therapeutic interventions.
Product Name:
Human PTGS2/COX-2 (Prostaglandin Endoperoxide Synthase 2) ELISA Kit
SKU:
HUES02800
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
