Description
Human PTGS2 Recombinant Protein (N-His)
Human PTGS2 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Phe187-Asn425, with an N-terminal His tag. Predicted molecular weight: 30.5 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Prostaglandin G/H synthase 2 (PTGS2) is a ~68 kDa protein. Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. For research use only.
The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Similarly catalyzes successive cyclooxygenation and peroxidation of dihomo-gamma-linoleate (DGLA, C20:3(n-6)) and eicosapentaenoate (EPA, C20:5(n-3)) to corresponding PGH1 and PGH3, the precursors of 1- and 3-series prostaglandins.
| Product Name: | Human PTGS2 Recombinant Protein (N-His) |
| SKU: | GXRP01482 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | PGH synthase 2, Prostaglandin G/H synthase 2, Cyclooxygenase-2, PTGS2, COX2, PGHS-2, PHS II, Prostaglandin-endoperoxide synthase 2, COX-2, Prostaglandin H2 synthase 2 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Phe187-Asn425 |
| Calculated MW: | 30.5 kDa |
| UniProt: | P35354 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
