The Human PTX3/TSG-14 (Pentraxin 3) ELISA Kit is a purpose-built assay designed for the quantitative measurement of Pentraxin 3 protein levels in various human biological samples. Pentraxin 3 (PTX3) is a critical fluid-phase pattern recognition receptor associated with diverse inflammatory processes and immune responses. It serves as a valuable diagnostic marker in various conditions, including infectious diseases, vascular disorders, and inflammatory responses. Accurate quantification of PTX3 is essential for elucidating its role in various pathological conditions and developing precise therapeutic interventions. Our ELISA kit is meticulously developed to provide outstanding sensitivity and specificity, enabling accurate and reproducible results for the quantitation of PTX3 levels. Manufactured under strict quality control measures, this kit ensures robust and reliable performance, offering a scientific tool of choice for both research investigations and clinical applications. Rely on the Human PTX3/TSG-14 ELISA Kit to achieve precise and dependable quantification of this vital biomarker for your scientific inquiries.
Product Name:
Human PTX 3/TSG-14 (Pentraxin 3) ELISA Kit
SKU:
AEES00117
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.12 pg/mL
Detection range:
6.86-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
