Human PURB Recombinant Protein (N-His) (GXRP04559)

Human PURB Recombinant Protein (N-His)

Human PURB Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Glu30-Val260, with an N-terminal His tag. Predicted molecular weight: 27.00 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Transcriptional regulator protein Pur-beta (PURB) is a ~33 kDa protein. Transcriptional regulator which can act as an activator or a repressor. For research use only.

Represses the transcription of ACTA2 in fibroblasts and smooth muscle cells via its ability to interact with the purine-rich strand of a MCAT- containing element in the 5' flanking region of the gene. Represses the transcription of MYOCD, capable of repressing all isoforms of MYOCD but the magnitude of the repressive effects is most notable for the SMC- specific isoforms. Promotes hepatic glucose production by activating the transcription of ADCY6, leading to cAMP accumulation, increased PKA activity, CREB activation, and increased transcription of PCK1 and G6PC genes. Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene.