SynonymsASC,CARD5,TMS1,Apoptosis-associated speck-like protein containing a CARD,Caspase recruitment domain-containing protein 5,Target of methylation-induced silencing 1
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates And Other Biological Fluids
SKU:
HUDL02473
UPC:
Product Type:
ELISA Kit
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ASC,CARD5,TMS1,Apoptosis-associated speck-like protein containing a CARD,Caspase recruitment domain-containing protein 5,Target of methylation-induced silencing 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit
The Human PYD (Pyrin domain) and CARD (Caspase activation and recruitment domain) Domain Containing Protein (PYCARD) ELISA Kit is specifically designed for the precise measurement of PYCARD levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers outstanding sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.PYCARD is a critical protein involved in inflammatory responses and immune regulation, playing a key role in conditions such as autoimmune diseases, inflammatory disorders, and infectious diseases.
Studying PYCARD levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies.Overall, the Human PYCARD ELISA Kit is a valuable tool for researchers looking to analyze PYCARD levels in human samples, providing reliable data for a better understanding of disease mechanisms and potential treatment strategies.
Product Name:
Human PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit
Product Code:
HUDL02473
Size:
96 Assays
Target:
PYCARD
Synonyms:
ASC, CARD5, TMS1, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, Target of methylation-induced silencing 1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
