Description
Human RAF1 Recombinant Protein (N-His)
Human RAF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Trp342-Ser619, with an N-terminal His tag. Predicted molecular weight: 34.12 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RAF proto-oncogene serine/threonine-protein kinase (RAF1) is a ~73 kDa protein. Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. For research use only.
RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity.
| Product Name: | Human RAF1 Recombinant Protein (N-His) |
| SKU: | GXRP00520 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | cRaf, RAF proto-oncogene serine/threonine-protein kinase, RAF1, Proto-oncogene c-RAF, RAF, Raf-1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Trp342-Ser619 |
| Calculated MW: | 34.12 kDa |
| UniProt: | P04049 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
