Description
Human RAPGEF1 Recombinant Protein (N-His)
Human RAPGEF1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Lys780-Asn1035, with an N-terminal His tag. Predicted molecular weight: 32.15 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. Rap guanine nucleotide exchange factor 1 (RAPGEF1) is a ~120 kDa protein. Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. For research use only.
Transduces signals from CRK to activate RAS. Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
| Product Name: | Human RAPGEF1 Recombinant Protein (N-His) |
| SKU: | GXRP02239 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Guanine nucleotide-releasing factor 2, RAPGEF1, GRF2, Protein C3G, CRK SH3-binding GNRP, Rap guanine nucleotide exchange factor 1 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Lys780-Asn1035 |
| Calculated MW: | 32.15 kDa |
| UniProt: | Q13905 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
