Description
Human RBCK1 Recombinant Protein (N-His)
Human RBCK1 Recombinant Protein (N-His) is a recombinant protein expressed in E. coli, spanning Met1-Gly156, with an N-terminal His tag. Predicted molecular weight: 19.94 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RanBP-type and C3HC4-type zinc finger-containing protein 1 (RBCK1) is a ~57 kDa protein. E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. For research use only.
Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways.
| Product Name: | Human RBCK1 Recombinant Protein (N-His) |
| SKU: | GXRP04674 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | RING finger protein 54, RNF54, RanBP-type and C3HC4-type zinc finger-containing protein 1, C20orf18, XAP4, Hepatitis B virus X-associated protein 4, UBCE7IP3, RBCK1, RING-type E3 ubiquitin transferase HOIL-1, HOIL-1, Heme-oxidized IRP2 ubiquitin ligase 1, XAP3, Ubiquitin-conjugating enzyme 7-interacting protein 3, HBV-associated factor 4 |
| Tag: | N-His |
| Expression Host: | E. coli |
| Protein Length: | Met1-Gly156 |
| Calculated MW: | 19.94 kDa |
| UniProt: | Q9BYM8 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
