Description
Human RBM8A Recombinant Protein (N-His-SUMO)
Human RBM8A Recombinant Protein (N-His-SUMO) is a recombinant protein expressed in E. coli, spanning Gly72-Arg152, with an N-terminal His-SUMO tag. Predicted molecular weight: 21.70 kDa. Purity: >90% as determined by SDS-PAGE. Supplied lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Applications: ELISA, immunogen, SDS-PAGE and Western blot. RNA-binding protein 8A (RBM8A) is a ~19 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. For research use only.
Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.
| Product Name: | Human RBM8A Recombinant Protein (N-His-SUMO) |
| SKU: | GXRP04826 |
| Size: | 50 µg, 100 µg, 1 mg |
| Species: | Human |
| Synonyms: | Ribonucleoprotein RBM8A, RNA-binding protein Y14, RNA-binding motif protein 8A, BOV-1, RBM8A, Binder of OVCA1-1, RBM8, RNA-binding protein 8A |
| Tag: | N-His-SUMO |
| Expression Host: | E. coli |
| Protein Length: | Gly72-Arg152 |
| Calculated MW: | 21.70 kDa |
| UniProt: | Q9Y5S9 |
| Applications: | ELISA, Immunogen, SDS-PAGE, WB |
| Purity: | >90% as determined by SDS-PAGE. |
| Form: | Lyophilized |
| Formulation: | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Reconstitution: | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. |
| Storage: | Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Shipping: | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Note: | For research use only. |
